phospho-CD244 (Tyr271) Polyclonal Antibody

$HeLa cell lysates probed with Anti-CD244(Tyr271) Polyclonal Antibody, Unconjugated (bs-5284R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.\\n$

HeLa cell lysates probed with Anti-CD244(Tyr271) Polyclonal Antibody, Unconjugated (bs-5284R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.\\n

MCF-7 cell lysates probed with Anti-CD244(Tyr271) Polyclonal Antibody, Unconjugated (bs-5284R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Pig
Horse
Rabbit

Overview
Catalog #	bs-5284R
Product Name	phospho-CD244 (Tyr271) Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human CD244 around the phosphorylation site of Tyr271
Modification Site	Tyr271
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51744
Swiss Prot	Q9BZW8
Subcellular location	Cell membrane
Synonyms	2B4; NAIL; Nmrk; NKR2B4; SLAMF4; Natural killer cell receptor 2B4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; h2B4; SLAM family member 4; Signaling lymphocytic activation molecule 4; CD244
Background	Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200