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Cell Biology

Cell Biology

Our Cell Biology Research Area supports the study of cellular architecture, signaling pathways, organelle activity, cell cycle control, and essential biological processes across a wide range of research workflows. This collection includes antibodies validated for use in WB, IHC, ICC, IF, FC, ELISA, and other common applications.



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Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Images provided by the Independent Validation Program (badge 29798).\\n\\nLane one: HeLa cell lysates irradiated with 25 Joules\/m2\\nUV-C Lane 2: Non-irradiated HeLa cell lysates probed with Rabbit Anti-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) polyclonal Antibody, Unconjugated (bs-2210R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2210R

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Unconjugated

Catalog #1: bs-2210R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: FITC

Catalog #1: bs-2210R-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Cy3

Catalog #1: bs-2210R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: HRP

Catalog #1: bs-0061R-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-594

Catalog #1: bs-2210R-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Cy5

Catalog #1: bs-2210R-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Cy7

Catalog #1: bs-2210R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Biotin

Catalog #1: bs-2210R-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Cy5.5

Catalog #1: bs-0061R-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5.5

Catalog #1: bs-2210R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: PE

Catalog #1: bs-0061R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE

Catalog #1: bs-2210R-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-350

Catalog #1: bs-2210R-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-405

Catalog #1: bs-2210R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-488

Catalog #1: bs-2210R-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-555

Catalog #1: bs-2210R-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-594

Catalog #1: bs-2210R-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-647

Catalog #1: bs-2210R-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-680

Catalog #1: bs-2210R-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: AbBy Fluor-750

Catalog #1: bs-2210R-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: APC

Catalog #1: bs-2210R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: APC-Cy5.5

Catalog #1: bs-2210R-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: APC-Cy7

Catalog #1: bs-2210R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: PE-Cy5

Catalog #1: bs-2210R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: PE-Cy5.5

Catalog #1: bs-2210R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: PE-Cy7

Catalog #1: bs-2210R-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: PerCP

Catalog #1: bs-2210R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2210R-RBITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: RBITC

Catalog #1: bs-0061R-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-350

Catalog #1: bs-0061R-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-405

Catalog #1: bs-0061R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-488

Catalog #1: bs-0061R-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-555

Catalog #1: bs-0061R-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-647

Catalog #1: bs-0061R-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-680

Catalog #1: bs-0061R-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-750

Catalog #1: bs-0061R-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC

Catalog #1: bs-0061R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy5.5

Catalog #1: bs-0061R-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy7

Catalog #1: bs-0061R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5

Catalog #1: bs-0061R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5.5

Catalog #1: bs-0061R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy7

Catalog #1: bs-0061R-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP

Catalog #1: bs-0061R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0061R-RBITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: RBITC