beta-Actin Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0061r-cy3-100uL |
| Product Name | beta-Actin Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat, Hamster |
| Predicted Reactivity | Dog, Sheep, Pig, Bee, Chicken, Cat, Rabbit, Fish, GuineaPig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | MAP conjugated synthetic peptide derived from human beta Actin |
| Immunogen Range | 1-200/375 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 60 |
| Swiss Prot | P60709 |
| Subcellular location | Cytoplasm |
| Synonyms | BRWS1; PS1TP5BP1; Actin, cytoplasmic 1; Beta-actin; ACTB |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Application Dilution | |
| WB | 1:5000-50000 |
| IF | 1:200-1000 |
| Flow-Cyt | 1μg/Test |
Frequently asked questions
Which applications is beta-Actin Polyclonal Antibody, Cy3 Conjugated (bs-0061R-Cy3) validated for, and at what dilution?
bs-0061R-Cy3 is validated for WB (1:5000-50000); IF (1:200-1000); Flow-Cyt (1μg/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does beta-Actin Polyclonal Antibody, Cy3 Conjugated (bs-0061R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat, Hamster. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Pig, Bee, Chicken, Cat, Rabbit, Fish, GuineaPig.
How should bs-0061R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise beta-Actin Polyclonal Antibody, Cy3 Conjugated (bs-0061R-Cy3)?
MAP conjugated synthetic peptide derived from human beta Actin Immunogen range: 1-200/375.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0061R-Cy3)?
Cy3 on bs-0061R-Cy3: excitation / emission 512,550nm/570,615nm.