HCV-NS4a Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0213r-hrp-100uL |
| Product Name | HCV-NS4a Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from HCV-NS4a |
| Immunogen Range | 1658-1711/3010 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | HCV-NS4a; Non-structural protein 4A; NS4A;POLG_HCVBK. |
| Background | Hepatitis C virus (HCV) is an enveloped, positive-strand RNA virus classified within the genus Hepacivirus in the family Flaviviridae. The nonsegmented HCV genome is 9.6-kb and encodes a long polyprotein that is cleaved by cellular and viral proteases into at least 10 distinct products. The N-terminal region of this polyprotein encodes the viral structural proteins: core and two envelope glycoproteins, E1 and E2. The remainder of the polyprotein encodes nonstructural (NS) proteins: p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. NS4A is the smallest NS protein. It encodes an N-terminal hydrophobic alpha-helix that anchors NS3-4A to cellular membranes, a middle cofactor peptide that forms a beta-strand and contributes to proper folding of the NS3 serine protease domain, and a C-terminal acidic region that forms an alpha-helix at low pH. |
| Application Dilution | |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is HCV-NS4a Polyclonal Antibody, HRP Conjugated (bs-0213R-HRP) validated for, and at what dilution?
bs-0213R-HRP is validated for ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HCV-NS4a Polyclonal Antibody, HRP Conjugated (bs-0213R-HRP) react with?
Tested reactivity: Virus.
How should bs-0213R-HRP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HCV-NS4a Polyclonal Antibody, HRP Conjugated (bs-0213R-HRP)?
KLH conjugated synthetic peptide derived from HCV-NS4a Immunogen range: 1658-1711/3010.