HCV Core protein Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0221R-Cy3-100uL |
| Product Name | HCV Core protein Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Virus, Others |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from HCV-Core |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P26664 |
| Subcellular location | Cytoplasm, Secreted, Cell membrane |
| Synonyms | Core protein p19; Hepatitis C Virus Core Antigen-C; HCVAg; HCV-Core protein;HCV core antigen; HCV core protein; Hepatitis C Virus core protein; capsid protein; polyprotein precursor; Core protein p21; Core protein p9. |
| Background | Core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. Modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit. Also regulates many host cellular functions such as signaling pathways and apoptosis. Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Plays an important role in virus-mediated cell transformation leading to hepatocellular carcinomas. Interacts with, and activates STAT3 leading to cellular transformation. May repress the promoter of p53, and sequester CREB3 and SP110 isoform3/Sp110b in the cytoplasm. Also represses cell cycle negative regulating factor CDKN1A, thereby interrupting an important check point of normal cell cycle regulation. Targets transcription factors involved in the regulation of inflammatory responses and in the immune response: suppresses NK-kappaB activation, and activates AP-1. Mediates apoptotic pathways throught association with TNF-type receptors TNFRSF1A and LTBR, although its effect on death receptors-induced apoptosis remains controvertial. Enhances TRAIL mediated apoptosis, suggesting that it might play a role in mediated apoptosis, suggesting that it might play a role in immune-mediated liver cell injury. Secreted core protein is able to bind C1QR1 at the T-cell surface, resulting in down-regulation of T-lymphocytes proliferation. May transactivate human MYC, Rous sarcoma virus LTR, and SV40 promoters. May suppress the human FOS and HIV-1 LTR activity. May alter lipid metabolism by interacting with hepatocellular proteins involved in lipid accumulation and storage. |
| Application Dilution | |
| WB | 1:500-1000 |
Frequently asked questions
Which applications is HCV Core protein Polyclonal Antibody, Cy3 Conjugated (bs-0221R-Cy3) validated for, and at what dilution?
bs-0221R-Cy3 is validated for WB (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HCV Core protein Polyclonal Antibody, Cy3 Conjugated (bs-0221R-Cy3) react with?
Tested reactivity: Human, Virus, Others.
How should bs-0221R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HCV Core protein Polyclonal Antibody, Cy3 Conjugated (bs-0221R-Cy3)?
KLH conjugated synthetic peptide derived from HCV-Core
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0221R-Cy3)?
Cy3 on bs-0221R-Cy3: excitation / emission 512,550nm/570,615nm.