Mouse Complement C5a Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0324r-bf350-100uL |
| Product Name | Mouse Complement C5a Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse C5a (725-770aa) |
| Immunogen Range | 950-1050/1680 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 15139 |
| Swiss Prot | P06684 |
| Subcellular location | Secreted |
| Synonyms | C5; He; C5b; Complement C5; Hemolytic complement; Hc |
| Background | Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
| Application Dilution | |
| WB | 1:50-200 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:200-400 |
| IF(ICC) | 1:500-1000 |
Frequently asked questions
Which applications is Mouse Complement C5a Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0324R-BF350) validated for, and at what dilution?
bs-0324R-BF350 is validated for WB (1:50-200); IF(IHC-P) (1:50-200); IF(IHC-F) (1:200-400); IF(ICC) (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mouse Complement C5a Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0324R-BF350) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-0324R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mouse Complement C5a Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0324R-BF350)?
KLH conjugated synthetic peptide derived from mouse C5a (725-770aa) Immunogen range: 950-1050/1680.
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-0324R-BF350)?
AbBy Fluor-350 on bs-0324R-BF350: excitation / emission 346nm/442nm.