H5N1 Matrix Protein 2 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0344R-FITC-100uL |
| Product Name | H5N1 Matrix Protein 2 Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will recognize many Influenza A virus strains |
| Reactivity | Virus |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Influenza A virus H5N1 Matrix Protein-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Avian influenza Matrix Protein-2; Influenza A virus H7N7 H9N2 H13N6 H16N3 H1N1 N2N1 H3N2 H2N2 |
| Background | Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is H5N1 Matrix Protein 2 Polyclonal Antibody, FITC Conjugated (bs-0344R-FITC) validated for, and at what dilution?
bs-0344R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does H5N1 Matrix Protein 2 Polyclonal Antibody, FITC Conjugated (bs-0344R-FITC) react with?
Tested reactivity: Virus.
How should bs-0344R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise H5N1 Matrix Protein 2 Polyclonal Antibody, FITC Conjugated (bs-0344R-FITC)?
KLH conjugated synthetic peptide derived from Influenza A virus H5N1 Matrix Protein-2
What are the excitation and emission wavelengths of the FITC conjugate (bs-0344R-FITC)?
FITC on bs-0344R-FITC: excitation / emission 494nm/518nm.