WNK4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0429R-Cy3-100uL |
| Product Name | WNK4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WNK4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 65266 |
| Subcellular location | Cytoplasm |
| Synonyms | PHA 2B; PHA2B; PRKWNK 4; PRKWNK4; Protein kinase lysine deficient 4; Protein kinase with no lysine 4; Serine/threonine protein kinase WNK4; WNK lysine deficient protein kinase 4; WNK4_HUMAN. |
| Background | Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+)channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+)secretion to maintain integrated homeostasis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is WNK4 Polyclonal Antibody, Cy3 Conjugated (bs-0429R-Cy3) validated for, and at what dilution?
bs-0429R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WNK4 Polyclonal Antibody, Cy3 Conjugated (bs-0429R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-0429R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WNK4 Polyclonal Antibody, Cy3 Conjugated (bs-0429R-Cy3)?
KLH conjugated synthetic peptide derived from human WNK4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0429R-Cy3)?
Cy3 on bs-0429R-Cy3: excitation / emission 512,550nm/570,615nm.