TSHR Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0460r-cy5.5-100uL |
| Product Name | TSHR Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TSHR |
| Immunogen Range | 501-600/764 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7253 |
| Swiss Prot | P16473 |
| Subcellular location | Cell membrane |
| Synonyms | LGR3; CHNG1; hTSHR-I; Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R; TSHR |
| Background | Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:100-500 |
Frequently asked questions
Which applications is TSHR Polyclonal Antibody, Cy5.5 Conjugated (BS-0460R-CY5-5) validated for, and at what dilution?
BS-0460R-CY5-5 is validated for WB (WB=1:500-2000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSHR Polyclonal Antibody, Cy5.5 Conjugated (BS-0460R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Pig, Horse, Chicken.
How should BS-0460R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSHR Polyclonal Antibody, Cy5.5 Conjugated (BS-0460R-CY5-5)?
KLH conjugated synthetic peptide derived from human TSHR Immunogen range: 501-600/764.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0460R-CY5-5)?
Cy5.5 on BS-0460R-CY5-5: excitation / emission 675nm/694nm.