Centromeric histone H3-like protein-2 Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0483R-BF750-100uL |
| Product Name | Centromeric histone H3-like protein-2 Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Plant |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Brassica juncea Centromeric histone H3-like protein-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1058 |
| Subcellular location | Nucleus |
| Synonyms | centromere-specic H3 variant protein; Centromeric histone H3-like protein 2; Centromeric histone H3-like protein-2; cenp-A; A4P1_BRAJU. |
| Background | Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely via acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1Alpha/Beta. The enzymes responsible for H3K9me3 formation are SUV39H1 and SUV39H2. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Centromeric histone H3-like protein-2 Antibody, AbBy Fluor-750 Conjugated (bs-0483R-BF750) validated for, and at what dilution?
bs-0483R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Centromeric histone H3-like protein-2 Antibody, AbBy Fluor-750 Conjugated (bs-0483R-BF750) react with?
Tested reactivity: Plant.
How should bs-0483R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Centromeric histone H3-like protein-2 Antibody, AbBy Fluor-750 Conjugated (bs-0483R-BF750)?
KLH conjugated synthetic peptide derived from Brassica juncea Centromeric histone H3-like protein-2
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-0483R-BF750)?
AbBy Fluor-750 on bs-0483R-BF750: excitation / emission 749nm/775nm.