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SOX2 Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Horse
  • Chicken
Overview
Catalog # bs-0523R-PE-Cy5-100uL
Product Name SOX2 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB FCM
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Horse, Chicken
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Transcription factor SOX-2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 20674
Swiss Prot P48432
Subcellular location Nucleus
Synonyms lcc; ysb; Sox-2; Transcription factor SOX-2; Sox2
Background Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is SOX2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-0523R-PE-Cy5) validated for, and at what dilution?

bs-0523R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SOX2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-0523R-PE-Cy5) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Horse, Chicken.

How should bs-0523R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SOX2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-0523R-PE-Cy5)?

KLH conjugated synthetic peptide derived from mouse Transcription factor SOX-2

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-0523R-PE-Cy5)?

PE-Cy5 on bs-0523R-PE-Cy5: excitation / emission 488nm/670nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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