Adiponectin receptor 2 Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0611R-Cy3-100uL |
| Product Name | Adiponectin receptor 2 Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may cross react with Adiponectin receptor 1 due to sequence similarity. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Pig, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Adiponectin receptor 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79602 |
| Swiss Prot | Q86V24 |
| Subcellular location | Cell membrane |
| Synonyms | PAQR2; ACDCR2; Adiponectin receptor protein 2; Progestin and adipoQ receptor family member II; ADIPOR2 |
| Background | Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Adiponectin receptor 2 Antibody, Cy3 Conjugated (bs-0611R-Cy3) validated for, and at what dilution?
bs-0611R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Adiponectin receptor 2 Antibody, Cy3 Conjugated (bs-0611R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Chicken.
How should bs-0611R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Adiponectin receptor 2 Antibody, Cy3 Conjugated (bs-0611R-Cy3)?
KLH conjugated synthetic peptide derived from human Adiponectin receptor 2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0611R-Cy3)?
Cy3 on bs-0611R-Cy3: excitation / emission 512,550nm/570,615nm.