ZNF300 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0628R-Biotin-100uL |
| Product Name | ZNF300 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
|
| Reactivity | Mouse |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZNF300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 91975 |
| Swiss Prot | Q96RE9 |
| Subcellular location | Nucleus |
| Synonyms | Zinc finger protein 3; ZNF3 |
| Background | ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is ZNF300 Polyclonal Antibody, Biotin Conjugated (bs-0628R-Biotin) validated for, and at what dilution?
bs-0628R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZNF300 Polyclonal Antibody, Biotin Conjugated (bs-0628R-Biotin) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-0628R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZNF300 Polyclonal Antibody, Biotin Conjugated (bs-0628R-Biotin)?
KLH conjugated synthetic peptide derived from human ZNF300