Collagen II Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0709R-Biotin-100uL |
| Product Name | Collagen II Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Rabbit |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Chicken, GuineaPig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Collagen II |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 1280 |
| Swiss Prot | P02458 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen; COL2A1 |
| Background | Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Collagen II Polyclonal Antibody, Biotin Conjugated (bs-0709R-Biotin) validated for, and at what dilution?
bs-0709R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Collagen II Polyclonal Antibody, Biotin Conjugated (bs-0709R-Biotin) react with?
Tested reactivity: Human, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Chicken, GuineaPig.
How should bs-0709R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen II Polyclonal Antibody, Biotin Conjugated (bs-0709R-Biotin)?
KLH conjugated synthetic peptide derived from human Collagen II