ADAMTS7(NT) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0733R-Cy5-100uL |
| Product Name | ADAMTS7(NT) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse ADAMTS7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 108153 |
| Swiss Prot | Q68SA9 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | ADAM-TS7; ADAMTS7B; A disintegrin and metalloproteinase with thrombospondin motifs 7; ADAM-TS 7; ADAMTS-7; COMPase; Adamts7 |
| Background | Metalloprotease that may play a role in the degradation of COMP. Binds 1 zinc ion per subunit. pH dependence: Optimum pH is between 7.5 and 9.5. [Subcellular location] Secreted, extracellular space, extracellular matrix (By similarity). Note=Also found associated with the external cell surface[Tissue specificity] Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. Up-regulated in articular cartilage and synovium from arthritis patients. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ADAMTS7(NT) Polyclonal Antibody, Cy5 Conjugated (bs-0733R-Cy5) validated for, and at what dilution?
bs-0733R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ADAMTS7(NT) Polyclonal Antibody, Cy5 Conjugated (bs-0733R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0733R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ADAMTS7(NT) Polyclonal Antibody, Cy5 Conjugated (bs-0733R-Cy5)?
KLH conjugated synthetic peptide derived from mouse ADAMTS7
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-0733R-Cy5)?
Cy5 on bs-0733R-Cy5: excitation / emission 625,650nm/670nm.