Mouse IgA Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0774R-Cy3-100uL |
| Product Name | Mouse IgA Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Mouse IgA protein purified from serum |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | affinity Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Background | Mouse IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain-linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids, and milk. Mucosal IgA plays a major role in host defense by neutralizing infectious agents at mucosal surfaces. The production is usually local and antigen-specific IgA-producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA-producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal-produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Mouse IgA Polyclonal Antibody, Cy3 Conjugated (bs-0774R-Cy3) validated for, and at what dilution?
bs-0774R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mouse IgA Polyclonal Antibody, Cy3 Conjugated (bs-0774R-Cy3) react with?
Tested reactivity: Mouse.
How should bs-0774R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mouse IgA Polyclonal Antibody, Cy3 Conjugated (bs-0774R-Cy3)?
Mouse IgA protein purified from serum
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0774R-Cy3)?
Cy3 on bs-0774R-Cy3: excitation / emission 512,550nm/570,615nm.