Angiopoietin-1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0800r-cy7-100uL |
| Product Name | Angiopoietin-1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Angiopoietin 1 |
| Immunogen Range | 276-375/498 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11600 |
| Subcellular location | Secreted |
| Synonyms | AGP 1; AGP1; AGPT; ANG 1; ANG1; Angiopoietin1; Angiogenin; ANGPT 1; ANGPT1; ANGPT-1; Angiopoietin-1; Angiopoietin 1; ANG-1; angiopoietin-1 isoform 1 precursor; ANGP1_HUMAN. |
| Background | Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Angiopoietin-1 Polyclonal Antibody, Cy7 Conjugated (bs-0800R-Cy7) validated for, and at what dilution?
bs-0800R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Angiopoietin-1 Polyclonal Antibody, Cy7 Conjugated (bs-0800R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig.
How should bs-0800R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Angiopoietin-1 Polyclonal Antibody, Cy7 Conjugated (bs-0800R-Cy7)?
KLH conjugated synthetic peptide derived from mouse Angiopoietin 1 Immunogen range: 276-375/498.
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-0800R-Cy7)?
Cy7 on bs-0800R-Cy7: excitation / emission 743nm/767nm.