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Alpha casein Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Bovine
  • Cow
Overview
Catalog # bs-0813R-BF750-100uL
Product Name Alpha casein Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Bovine, Cow
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source Casein protein from bovine milk
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Secreted, Cell membrane
Synonyms NULL
Background Casein is the name for a family of related phosphoproteins. These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive, to a binder for safety matches. As a food source, casein supplies amino acids; carbohydrates; and two inorganic elements, calcium and phosphorus. Casein from bovine milk is a phosphoprotein. There are four main types of casein which make up approximately 80% of the total protein in bovine milk: _-s1 casein, _-s2 casein, _-casein, and _-casein. Casein is proposed to be the main protective constituent in milk. 
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is Alpha casein Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0813R-BF750) validated for, and at what dilution?

bs-0813R-BF750 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Alpha casein Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0813R-BF750) react with?

Tested reactivity: Bovine, Cow.

How should bs-0813R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Alpha casein Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0813R-BF750)?

Casein protein from bovine milk

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-0813R-BF750)?

AbBy Fluor-750 on bs-0813R-BF750: excitation / emission 749nm/775nm.

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