phospho-AKT (Ser473) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0876r-Cy5.5-100uL |
| Product Name | phospho-AKT (Ser473) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473 |
| Modification Site | Ser473 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 24185 |
| Swiss Prot | P47196 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1 |
| Background | AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is phospho-AKT (Ser473) Polyclonal Antibody, Cy5.5 Conjugated (BS-0876R-CY5-5) validated for, and at what dilution?
BS-0876R-CY5-5 is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-AKT (Ser473) Polyclonal Antibody, Cy5.5 Conjugated (BS-0876R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-0876R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-AKT (Ser473) Polyclonal Antibody, Cy5.5 Conjugated (BS-0876R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0876R-CY5-5)?
Cy5.5 on BS-0876R-CY5-5: excitation / emission 675nm/694nm.