GFP Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0890R-APC-Cy5.5-100uL |
| Product Name | GFP Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Pig, Tags |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P42212 |
| Synonyms | Green fluorescence protein; GRP; GFP TAG, GFP-TAG. |
| Background | Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GFP Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-0890R-APC-CY5-5) validated for, and at what dilution?
BS-0890R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GFP Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-0890R-APC-CY5-5) react with?
Tested reactivity: Pig, Tags.
How should BS-0890R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GFP Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-0890R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-0890R-APC-CY5-5)?
APC-Cy5.5 on BS-0890R-APC-CY5-5: excitation / emission 650nm/694nm.