PDX1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0923R-Cy3-100uL |
| Product Name | PDX1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Pig, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse PDX1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 18609 |
| Swiss Prot | P52946 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Ipf1; IDX-1; IPF-1; Mody4; STF-1; pdx-1; Pancreas/duodenum homeobox protein 1; Insulin promoter factor 1; Islet/duodenum homeobox 1; Somatostatin-transactivating factor 1; Pdx1 |
| Background | Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PDX1 Polyclonal Antibody, Cy3 Conjugated (bs-0923R-Cy3) validated for, and at what dilution?
bs-0923R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDX1 Polyclonal Antibody, Cy3 Conjugated (bs-0923R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Pig, Chicken.
How should bs-0923R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDX1 Polyclonal Antibody, Cy3 Conjugated (bs-0923R-Cy3)?
KLH conjugated synthetic peptide derived from mouse PDX1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0923R-Cy3)?
Cy3 on bs-0923R-Cy3: excitation / emission 512,550nm/570,615nm.