PRRSV Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10043R-Biotin-100uL |
| Product Name | PRRSV Polyclonal Antibody, Biotin Conjugated |
| Applications |
IHC-P
IHC-F
|
| Reactivity | Virus, Others |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | Derived from an inactive PRRSV vaccine strain NVDC-JXA1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Synonyms | Porcine reproductive and respiratory syndrome, PRRS. |
| Background | PRRSV belongs to the family Arteriviridae in the genus Arterivirus, order Nidovirales. This group also includes lactate dehydrogenase-elevating virus (LDV) of mice, equine arteritis virus (EAV) and simian hemorrhagic fever virus (SHFV). PRRSV is a small, enveloped virus of 45-70 nm in diameter. Its genome consists of a polyadenylated, single-stranded, non-segmented, positivesense RNA polycystronic molecule of 15.1-15.5 kb. It contains, in the 5 to 3 direction, a 5 leader sequence, at least 10 open reading frames (ORF1a, ORF1b and ORFs 2a, 2b, 3, 4, 5, 5a, 6 and 7) and a 3 non-translated region. ORF1a and ORF1b are located directly downstream of the 5 leader and encode a large replicase polyprotein, which is thought to be autoproteolytically cleaved into at least 13 smaller non-structural proteins including a viral RNA-dependent RNA polymerase. |
| Application Dilution | |
| IHC-P | 1:500-1000 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is PRRSV Polyclonal Antibody, Biotin Conjugated (bs-10043R-Biotin) validated for, and at what dilution?
bs-10043R-Biotin is validated for IHC-P (1:500-1000); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PRRSV Polyclonal Antibody, Biotin Conjugated (bs-10043R-Biotin) react with?
Tested reactivity: Virus, Others.
How should bs-10043R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PRRSV Polyclonal Antibody, Biotin Conjugated (bs-10043R-Biotin)?
Derived from an inactive PRRSV vaccine strain NVDC-JXA1