AAV5 capsid protein VP1 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10180R-HRP-100uL |
| Product Name | AAV5 capsid protein VP1 Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Specificity | This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13 |
| Reactivity | Virus, Others |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | capsid protein Adeno-associated 5 virus; capsid protein [Adeno-associated virus - 5]; capsid protein AAV5; Parvovirus coat protein VP1; capsid protein. |
| Background | Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is AAV5 capsid protein VP1 Polyclonal Antibody, HRP Conjugated (bs-10180R-HRP) validated for, and at what dilution?
bs-10180R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AAV5 capsid protein VP1 Polyclonal Antibody, HRP Conjugated (bs-10180R-HRP) react with?
Tested reactivity: Virus, Others.
How should bs-10180R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AAV5 capsid protein VP1 Polyclonal Antibody, HRP Conjugated (bs-10180R-HRP)?
KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1