alpha smooth muscle Actin Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10196R-PE-Cy5.5-100uL |
| Product Name | alpha smooth muscle Actin Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Specificity | Alpha, Beta and Gamma Actins |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rabbit |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 70 |
| Swiss Prot | P68032 |
| Subcellular location | Cytoplasm |
| Synonyms | Actin, alpha skeletal muscle; ACTA1; Alpha-actin-1; Actin, alpha cardiac muscle 1; ACTC1; Alpha-cardiac actin; Actin, aortic smooth muscle; ACTA2; Alpha-actin-2; Actin, gamma-enteric smooth muscle; ACTG2; Actin, cytoplasmic 2; ACTG1; Actin, cytoplasmic 1; Gamma-actin; ACTB; Beta-actin; |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is alpha smooth muscle Actin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-10196R-PE-CY5-5) validated for, and at what dilution?
BS-10196R-PE-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does alpha smooth muscle Actin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-10196R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.
How should BS-10196R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise alpha smooth muscle Actin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-10196R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-10196R-PE-CY5-5)?
PE-Cy5.5 on BS-10196R-PE-CY5-5: excitation / emission 488nm/694nm.