TLR2 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1019r-apc-cy55-100uL |
| Product Name | TLR2 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TLR2 (cytoplasmic domain) |
| Immunogen Range | 701-784/784 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7097 |
| Swiss Prot | O60603 |
| Subcellular location | Cell membrane |
| Synonyms | TIL4; CD282; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; TLR2 |
| Background | Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TLR2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-1019R-APC-CY5-5) validated for, and at what dilution?
BS-1019R-APC-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TLR2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-1019R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-1019R-APC-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TLR2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-1019R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human TLR2 (cytoplasmic domain) Immunogen range: 701-784/784.
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-1019R-APC-CY5-5)?
APC-Cy5.5 on BS-1019R-APC-CY5-5: excitation / emission 650nm/694nm.