H3N1 hemagglutinin Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10320R-Cy7-100uL |
| Product Name | H3N1 hemagglutinin Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from hemagglutinin [Influenza A virus A/environment/North Dakota/NWRC185233-06/2007(H3N1)] |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | HA; Influenza A virus H3N1; hemagglutinin; H3N1; H3N1 hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain; K9URT3_9INFA. |
| Background | Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is H3N1 hemagglutinin Polyclonal Antibody, Cy7 Conjugated (bs-10320R-Cy7) validated for, and at what dilution?
bs-10320R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does H3N1 hemagglutinin Polyclonal Antibody, Cy7 Conjugated (bs-10320R-Cy7) react with?
Tested reactivity: Virus.
How should bs-10320R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise H3N1 hemagglutinin Polyclonal Antibody, Cy7 Conjugated (bs-10320R-Cy7)?
KLH conjugated synthetic peptide derived from hemagglutinin [Influenza A virus A/environment/North Dakota/NWRC185233-06/2007(H3N1)]
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-10320R-Cy7)?
Cy7 on bs-10320R-Cy7: excitation / emission 743nm/767nm.