Bovine Fibrinogen Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-10361R-BF350-100uL |
| Product Name | Bovine Fibrinogen Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Cow |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | Fibrinogen from bovine plasma |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 522039 |
| Subcellular location | Secreted |
| Synonyms | FGA; FGA protein; FGB; FGG; Fib2; Fibrin alpha chain ;Fibrinogen A alpha polypeptide; Fibrinogen A alpha polypeptide chain; Fibrinogen alpha chain; Fibrinogen B alpha polypeptide; Fibrinogen beta chain; Fibrinogen G alpha polypeptide; Fibrinogen gamma chain; MGC104327; MGC119422; MGC119423; MGC119425; MGC120405. |
| Background | Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel A. et al, and Rapold H.J. et al). All these facts make fibrinogen an important parameter in the diagnosis of cardiovascular diseases. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Bovine Fibrinogen Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-10361R-BF350) validated for, and at what dilution?
bs-10361R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-10361R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bovine Fibrinogen Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-10361R-BF350)?
Fibrinogen from bovine plasma
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-10361R-BF350)?
AbBy Fluor-350 on bs-10361R-BF350: excitation / emission 346nm/442nm.