TSPAN12 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10425R-Cy7-100uL |
| Product Name | TSPAN12 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TSPAN12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23554 |
| Swiss Prot | O95859 |
| Subcellular location | Cell membrane |
| Synonyms | EVR5; NET2; NET-2; TM4SF12; Tetraspanin-12; Tspan-12; Tetraspan NET-2; Transmembrane 4 superfamily member 12; TSPAN12; UNQ774/PRO1568 |
| Background | Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TSPAN12 Polyclonal Antibody, Cy7 Conjugated (bs-10425R-Cy7) validated for, and at what dilution?
bs-10425R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSPAN12 Polyclonal Antibody, Cy7 Conjugated (bs-10425R-Cy7) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-10425R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSPAN12 Polyclonal Antibody, Cy7 Conjugated (bs-10425R-Cy7)?
KLH conjugated synthetic peptide derived from human TSPAN12
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-10425R-Cy7)?
Cy7 on bs-10425R-Cy7: excitation / emission 743nm/767nm.