VAMP1+VAMP2+VAMP3 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10427R-Cy3-100uL |
| Product Name | VAMP1+VAMP2+VAMP3 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VAMP-1+2+3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Vesicle Associated Membrane Protein 1; DKFZp686H12131; SYB 1; SYB1; Synaptobrevin 1; Synaptobrevin1; Synaptobrevin-1; VAMP 1; VAMP-1; Vesicle associated membrane protein 1; Vesicle associated membrane protein 1 synaptobrevin 1; VAMP1_HUMAN; Vesicle-associated membrane protein 1; Vesicle Associated Membrane Protein 2; FLJ11460; RATVAMPB; RATVAMPIR; SYB; SYB2; Synaptobrevin 2; VAMP 2; VAMP-2; Vesicle associated membrane protein 2; Vesicle-associated membrane protein 2; VAMP2_HUMAN; Synaptobrevin-2; Vesicle Associated Membrane Protein 3; VAMP-3; VAMP 3; VAMP3; CEB; Cellubrevin; Synaptobrevin 3; Synaptobrevin-3; MGC108618; Vamp3; D130027G05Rik; VAMP3_HUMAN; Vesicle-associated membrane protein 3. |
| Background | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VAMP1+VAMP2+VAMP3 Polyclonal Antibody, Cy3 Conjugated (bs-10427R-Cy3) validated for, and at what dilution?
bs-10427R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VAMP1+VAMP2+VAMP3 Polyclonal Antibody, Cy3 Conjugated (bs-10427R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-10427R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VAMP1+VAMP2+VAMP3 Polyclonal Antibody, Cy3 Conjugated (bs-10427R-Cy3)?
KLH conjugated synthetic peptide derived from human VAMP-1+2+3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-10427R-Cy3)?
Cy3 on bs-10427R-Cy3: excitation / emission 512,550nm/570,615nm.