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ADRB3 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Horse
  • Rabbit
Overview
Catalog # bs-10921R-Cy5.5-100uL
Product Name ADRB3 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Horse, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ADRB3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 155
Swiss Prot P13945
Subcellular location Cell membrane
Synonyms BETA3AR; Beta-3 adrenergic receptor; Beta-3 adrenoreceptor; Beta-3 adrenoceptor; ADRB3; ADRB3R; B3AR
Background Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ADRB3 Polyclonal Antibody, Cy5.5 Conjugated (BS-10921R-CY5-5) validated for, and at what dilution?

BS-10921R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADRB3 Polyclonal Antibody, Cy5.5 Conjugated (BS-10921R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse, Rabbit.

How should BS-10921R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADRB3 Polyclonal Antibody, Cy5.5 Conjugated (BS-10921R-CY5-5)?

KLH conjugated synthetic peptide derived from human ADRB3

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-10921R-CY5-5)?

Cy5.5 on BS-10921R-CY5-5: excitation / emission 675nm/694nm.

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