TMP21 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11262R-Cy5.5-100uL |
| Product Name | TMP21 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | KO-Validated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TMP21 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | p23; p24 family protein delta 1; p24delta; p24delta1; S31I125; S31III125; TMED 10; TMED10; Tmp 21 I; Tmp 21; Tmp 21 I; Transmembrane emp24 domain containing protein 10; Transmembrane emp24-like trafficking protein 10 yeast; Transmembrane protein Tmp21; Transmembrane trafficking protein 21kD; 1110014C03Rik; 21 kDa transmembrane trafficking protein; MGC102351; TMEDA_HUMAN. |
| Background | MP21 is a member of the EMP24/GP25L/p24 cargo family of proteins that regulates vesicular trafficking in the early secretory pathway. TMP21 is a ubiquitously expressed single-pass type I membrane protein localizing to the Golgi cisternae and the plasma membrane. It contains one GOLD (Golgi dynamics) domain and participates in protein transport and quality control between the endoplasmic reticulum (ER) and the Golgi complex. In addition, TMP21 is a component of the heteromeric secretase complex (or Presenilin complex) and functions to regulate the ?secretase activity. Two isoforms, namely TMP21-I and TMP21-II, exist for this protein. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TMP21 Polyclonal Antibody, Cy5.5 Conjugated (BS-11262R-CY5-5) validated for, and at what dilution?
BS-11262R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TMP21 Polyclonal Antibody, Cy5.5 Conjugated (BS-11262R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Chicken, Rabbit.
How should BS-11262R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TMP21 Polyclonal Antibody, Cy5.5 Conjugated (BS-11262R-CY5-5)?
KLH conjugated synthetic peptide derived from human TMP21
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11262R-CY5-5)?
Cy5.5 on BS-11262R-CY5-5: excitation / emission 675nm/694nm.