5-HT Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1126R-Cy5-100uL |
| Product Name | 5-HT Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat, Others |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated to 5-HT |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Serotonin; 5-hydroxytryptamine; 3-2-Aminoethyl-5-hydroxyindole hydrochloride; 5-HT, 5-Hydroxytryptamine hydrochloride. |
| Background | Serotonin (pronounced) (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system (CNS) and enterochromaffin cells in the gastrointestinal tract of animals including humans. Serotonin is also found in many mushrooms and plants, including fruits and vegetables. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is 5-HT Polyclonal Antibody, Cy5 Conjugated (bs-1126R-Cy5) validated for, and at what dilution?
bs-1126R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5-HT Polyclonal Antibody, Cy5 Conjugated (bs-1126R-Cy5) react with?
Tested reactivity: Rat, Others.
How should bs-1126R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5-HT Polyclonal Antibody, Cy5 Conjugated (bs-1126R-Cy5)?
KLH conjugated to 5-HT
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-1126R-Cy5)?
Cy5 on bs-1126R-Cy5: excitation / emission 625,650nm/670nm.