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5-HT Polyclonal Antibody, Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat
  • Others
Overview
Catalog # bs-1126R-Cy7-100uL
Product Name 5-HT Polyclonal Antibody, Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat, Others
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated to 5-HT
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms Serotonin; 5-hydroxytryptamine; 3-2-Aminoethyl-5-hydroxyindole hydrochloride; 5-HT, 5-Hydroxytryptamine hydrochloride.
Background Serotonin (pronounced) (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system (CNS) and enterochromaffin cells in the gastrointestinal tract of animals including humans. Serotonin is also found in many mushrooms and plants, including fruits and vegetables.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is 5-HT Polyclonal Antibody, Cy7 Conjugated (bs-1126R-Cy7) validated for, and at what dilution?

bs-1126R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does 5-HT Polyclonal Antibody, Cy7 Conjugated (bs-1126R-Cy7) react with?

Tested reactivity: Rat, Others.

How should bs-1126R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise 5-HT Polyclonal Antibody, Cy7 Conjugated (bs-1126R-Cy7)?

KLH conjugated to 5-HT

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-1126R-Cy7)?

Cy7 on bs-1126R-Cy7: excitation / emission 743nm/767nm.

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