TSARG2/SPATA4 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11309R-Cy5.5-100uL |
| Product Name | TSARG2/SPATA4 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TSARG2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Testis and spermatogenesis related gene 3; TSARG 2; TSARG-2; TSARG2; SPAT4_HUMAN. |
| Background | The testis spermatocyte apoptosis-related gene 2 protein (TSARG2, also designated spermatogenesis associated-4 or SPATA4) is involved in spermatogenesis. TSARG2 is specifically expressed in spermatogonia and spermatocytes of the seminiferous tubules, and it localizes to the nucleus. The predicted molecular weight of TSARG2 ranges depending on the species. TSARG2 is significantly upregulated in cryptorchidism and therefore, is a testis-specific apoptosis candidate oncogene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TSARG2/SPATA4 Polyclonal Antibody, Cy5.5 Conjugated (BS-11309R-CY5-5) validated for, and at what dilution?
BS-11309R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSARG2/SPATA4 Polyclonal Antibody, Cy5.5 Conjugated (BS-11309R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Pig, Rabbit.
How should BS-11309R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSARG2/SPATA4 Polyclonal Antibody, Cy5.5 Conjugated (BS-11309R-CY5-5)?
KLH conjugated synthetic peptide derived from human TSARG2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11309R-CY5-5)?
Cy5.5 on BS-11309R-CY5-5: excitation / emission 675nm/694nm.