Acetoacetyl-CoA synthetase Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11383R-BF750-100uL |
| Product Name | Acetoacetyl-CoA synthetase Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AACS |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 65985 |
| Subcellular location | Cytoplasm |
| Synonyms | Acetoacetyl-CoA synthetase; ACSF1; Acyl-CoA synthetase family member 1; Protein sur-5 homolog; AACS_HUMAN. |
| Background | ACSF1 is a 672 amino acid protein belonging to the ATP-dependent AMP-binding enzyme family. Encoded by a gene that maps to human chromosome 12q24.31, ACSF1 is highly expressed in kidney, heart and brain, and shows similar neural expression as HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase). Existing as three alternatively spliced isoforms, ACSF1 participates in ATP binding, ligase activity, acetoacetate-CoA ligase activity and nucleotide binding. The ACSF1 promoter is a known PPAR?target gene, with the nuclear receptor recruited to the ACSF1 promoter by direct interaction with stimulating protein-1 (Sp1). ACSF1 activates acetoacetate and is highly regulated by modulators that affect HMGCR and cholesterol biosynthesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Acetoacetyl-CoA synthetase Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11383R-BF750) validated for, and at what dilution?
bs-11383R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11383R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Acetoacetyl-CoA synthetase Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11383R-BF750)?
KLH conjugated synthetic peptide derived from human AACS
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11383R-BF750)?
AbBy Fluor-750 on bs-11383R-BF750: excitation / emission 749nm/775nm.