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PCTAIRE2 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Sheep

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Chicken
  • Rabbit
Overview
Catalog # bs-11574R-Cy7-100uL
Product Name PCTAIRE2 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Sheep
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Chicken, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PCTAIRE2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm, Nucleus
Synonyms PCTAIRE-2; PCTAIRE-mot protein kinase 2; PCTK2; Protein kinase cdc2-related PCTAIRE-2; Serine/threonine-protein kinase PCTAIRE-2; 6430598J10Rik; EC 2.7.1.37; MGC25109; CDK17_HUMAN.
Background Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with cyclins to phosphorylate key substrates involved in cell cycle progression. Another family of proteins, Cdk inhibitors, also play a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Members of the Cdk family include Cdk2Cdk8, PCTAIRE-13, PITALRE and PITSLRE. PCTAIRE-1, PCTAIRE-2 and PCTAIRE-3 comprise a subfamily of cdc2-related serine/threonine kinases. PCTAIRE-1, which is expressed primarily in mammalian brain, interacts with a variety of proteins and is thought to be part of a multiple signal transduction cascade. PCTAIRE-2, also expressed in brain, may be important in terminally differentiated neurons.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PCTAIRE2 Polyclonal Antibody, Cy7 Conjugated (bs-11574R-Cy7) validated for, and at what dilution?

bs-11574R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PCTAIRE2 Polyclonal Antibody, Cy7 Conjugated (bs-11574R-Cy7) react with?

Tested reactivity: Sheep. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat, Dog, Cow, Chicken, Rabbit.

How should bs-11574R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PCTAIRE2 Polyclonal Antibody, Cy7 Conjugated (bs-11574R-Cy7)?

KLH conjugated synthetic peptide derived from human PCTAIRE2

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-11574R-Cy7)?

Cy7 on bs-11574R-Cy7: excitation / emission 743nm/767nm.

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