WBP2 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11625R-APC-Cy7-100uL |
| Product Name | WBP2 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WBP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | MGC18269; WBP 2; WBP-2; Wbp2; WBP2_HUMAN; WW domain binding protein 2; WW domain-binding protein 2. |
| Background | WW domain-binding protein 2 (WBP2) is a 261 amino acid protein expressed in most tissues. The WW domain is composed of 38 to 40 semi-conserved amino acids and is shared by various groups of proteins, including structural, regulatory and signaling proteins. The domain mediates protein-protein interactions through the binding of polyproline ligands. WBP2 binds to the WW domain of Yes-associated protein (YAP), WW domain containing E3 ubiquitin protein ligase 1 (AIP5) and WW domain containing E3 ubiquitin protein ligase 2 (AIP2). The gene encoding WBP2 is located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes, some of which are involved in tumor suppression and in the pathogenesis of Li-Fraumeni syndrome, early onset breast cancer and a predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is WBP2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-11625R-APC-Cy7) validated for, and at what dilution?
bs-11625R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WBP2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-11625R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse.
How should bs-11625R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WBP2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-11625R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human WBP2
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-11625R-APC-Cy7)?
APC-Cy7 on bs-11625R-APC-Cy7: excitation / emission 650nm/780nm.