APBB3 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11637R-Cy5.5-100uL |
| Product Name | APBB3 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APBB3/FE65L2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10307 |
| Swiss Prot | O95704 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Amyloid beta A4 precursor protein binding family B member 3; Amyloid beta A4 precursor protein-binding family B member 3; amyloid beta precursor protein binding family B member 3; amyloid precursor interacting protein; Apbb3; APBB3_HUMAN; Fe65 like protein 2; FE65L2; Protein Fe65-like 2; SRA. |
| Background | Fe65L2 is a 486 amino acid protein that contains one WW domain and two PID domains. Binding to the intracellular domain of the -Amyloid precursor protein, Fe65L2 is thought to modulate the internalization and, therefore, the accessibility and function of -Amyloid. Via its ability to control the intracellular accumulation of -Amyloid, Fe65L2 is thought to play a role in the pathogenesis of Alzheimer's disease. Fe65L2 exists as four alternatively spliced isoforms designated isoform I, isoform II, isoform III and isoform IV. Fe65L2 interacts with Amyloid-like protein and is encoded by a gene located on human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is APBB3 Polyclonal Antibody, Cy5.5 Conjugated (BS-11637R-CY5-5) validated for, and at what dilution?
BS-11637R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APBB3 Polyclonal Antibody, Cy5.5 Conjugated (BS-11637R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep.
How should BS-11637R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APBB3 Polyclonal Antibody, Cy5.5 Conjugated (BS-11637R-CY5-5)?
KLH conjugated synthetic peptide derived from human APBB3/FE65L2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11637R-CY5-5)?
Cy5.5 on BS-11637R-CY5-5: excitation / emission 675nm/694nm.