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5HT4 Receptor Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • GuineaPig
Overview
Catalog # bs-12054R-Biotin-100uL
Product Name 5HT4 Receptor Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-F
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken, GuineaPig
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 5HT4 Receptor
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 3360
Swiss Prot Q13639
Subcellular location Cytoplasm, Cell membrane
Synonyms 5 HT 4; 5 HT4; 5 HT4R; 5 hydroxytryptamine 4 receptor; 5 hydroxytryptamine receptor 4; 5 hydroxytryptamine serotonin receptor 4; 5 hydroxytryptamine4 receptor; 5-HT-4; 5-ht4; 5-HT4R; 5H4; 5HT 4; 5HT4; 5ht4 receptor; 5HT4R; Cardiac 5 HT4 receptor; HTR 4; HTR4; serotonin 4 receptor; Serotonin 5 HT4 receptor; serotonin 5-ht-4 receptor; serotonin 5-ht-4a. Serotonin receptor 4; 5HT4R_HUMAN.
Background This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500

Frequently asked questions

Which applications is 5HT4 Receptor Polyclonal Antibody, Biotin Conjugated (bs-12054R-Biotin) validated for, and at what dilution?

bs-12054R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does 5HT4 Receptor Polyclonal Antibody, Biotin Conjugated (bs-12054R-Biotin) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, GuineaPig.

How should bs-12054R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise 5HT4 Receptor Polyclonal Antibody, Biotin Conjugated (bs-12054R-Biotin)?

KLH conjugated synthetic peptide derived from human 5HT4 Receptor