5HT4 Receptor Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12054R-Cy5.5-100uL |
| Product Name | 5HT4 Receptor Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken, GuineaPig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 5HT4 Receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3360 |
| Swiss Prot | Q13639 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 5 HT 4; 5 HT4; 5 HT4R; 5 hydroxytryptamine 4 receptor; 5 hydroxytryptamine receptor 4; 5 hydroxytryptamine serotonin receptor 4; 5 hydroxytryptamine4 receptor; 5-HT-4; 5-ht4; 5-HT4R; 5H4; 5HT 4; 5HT4; 5ht4 receptor; 5HT4R; Cardiac 5 HT4 receptor; HTR 4; HTR4; serotonin 4 receptor; Serotonin 5 HT4 receptor; serotonin 5-ht-4 receptor; serotonin 5-ht-4a. Serotonin receptor 4; 5HT4R_HUMAN. |
| Background | This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is 5HT4 Receptor Polyclonal Antibody, Cy5.5 Conjugated (BS-12054R-CY5-5) validated for, and at what dilution?
BS-12054R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5HT4 Receptor Polyclonal Antibody, Cy5.5 Conjugated (BS-12054R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, GuineaPig.
How should BS-12054R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5HT4 Receptor Polyclonal Antibody, Cy5.5 Conjugated (BS-12054R-CY5-5)?
KLH conjugated synthetic peptide derived from human 5HT4 Receptor
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12054R-CY5-5)?
Cy5.5 on BS-12054R-CY5-5: excitation / emission 675nm/694nm.