TRAIL Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1214R-Cy3-100uL |
| Product Name | TRAIL Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRAIL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8743 |
| Swiss Prot | P50591 |
| Subcellular location | Cell membrane |
| Synonyms | TL2; APO2L; CD253; TRAIL; Apo-2L; Tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis-inducing ligand; Protein TRAIL; TNFSF10 |
| Background | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TRAIL Polyclonal Antibody, Cy3 Conjugated (bs-1214R-Cy3) validated for, and at what dilution?
bs-1214R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRAIL Polyclonal Antibody, Cy3 Conjugated (bs-1214R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1214R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRAIL Polyclonal Antibody, Cy3 Conjugated (bs-1214R-Cy3)?
KLH conjugated synthetic peptide derived from human TRAIL
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1214R-Cy3)?
Cy3 on bs-1214R-Cy3: excitation / emission 512,550nm/570,615nm.