ZFP36L1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12254R-Cy5-100uL |
| Product Name | ZFP36L1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZFP36L1/ERF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 677 |
| Subcellular location | Nucleus |
| Synonyms | Berg36; BRF1; Butyrate response factor 1; C3H1 type-like 1; cMG1; Early response factor Berg36; EGF response factor 1; EGF-response factor 1; ERF 1; ERF-1; ERF1; Protein TIS11B; RNF162B; TIS11B; TISB_HUMAN; ZFP36L1; Zinc finger protein 36; Zinc finger protein 36 C3H type like 1; zinc finger protein C3H type 36 like 1. |
| Background | TIS11B is a member of the tristetraprolin family. Tristetraprolin (TTP), or TIS11, is a zinc-binding protein encoded by the immediate-early response gene, Zfp-36. TIS11B, a relative of TTP, localizes to the nucleus and may function as a transcription factor involved in regulating the growth factor response. It is an evolutionarily conserved protein containing two C3H1-type zinc fingers and a repeating cys-his motif. TIS11B is an mRNA binding protein and is known to interact with the 3?-untranslated region of VEGF mRNA, thereby decreasing its stability. This suggests that TIS11B is a potential target in antiangiogenic therapy. In addition, TIS11B may also be an important regulator of myogenesis, as its expression is upregulated during murine myoblast differentiation. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZFP36L1 Polyclonal Antibody, Cy5 Conjugated (bs-12254R-Cy5) validated for, and at what dilution?
bs-12254R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZFP36L1 Polyclonal Antibody, Cy5 Conjugated (bs-12254R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse.
How should bs-12254R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZFP36L1 Polyclonal Antibody, Cy5 Conjugated (bs-12254R-Cy5)?
KLH conjugated synthetic peptide derived from human ZFP36L1/ERF1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12254R-Cy5)?
Cy5 on bs-12254R-Cy5: excitation / emission 625,650nm/670nm.