ABL2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1230R-Cy3-100uL |
| Product Name | ABL2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ABL2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27 |
| Subcellular location | Cytoplasm |
| Synonyms | Abelson murine leukemia viral oncogene homolog 2; Abelson related gene protein; Tyrosine kinase ARG; ABLL;ARG;Tyrosine protein kinase ABL2; v abl Abelson murine leukemia viral oncogene homolog 2; ABL2_HUMAN; Abelson tyrosine-protein kinase 2; Tyrosine-protein kinase ARG. |
| Background | ABL2 (or ARG) is a nonreceptor cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of ABL1 and ABL2 includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. It is involved in translocation with the ETV6 gene in human leukemia and has an altered expression in several human carcinomas. Two isoforms of ABL2 with different N-termini (1A and 1B) have been identified. The C-terminal domain of ABL2 contains two F-actin-binding sequences that perform a number of actions related to cell morphology and motility by interacting with actin filaments. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ABL2 Polyclonal Antibody, Cy3 Conjugated (bs-1230R-Cy3) validated for, and at what dilution?
bs-1230R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ABL2 Polyclonal Antibody, Cy3 Conjugated (bs-1230R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Horse, Rabbit.
How should bs-1230R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ABL2 Polyclonal Antibody, Cy3 Conjugated (bs-1230R-Cy3)?
KLH conjugated synthetic peptide derived from human ABL2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1230R-Cy3)?
Cy3 on bs-1230R-Cy3: excitation / emission 512,550nm/570,615nm.