ApoM Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12501R-Cy5.5-100uL |
| Product Name | ApoM Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ApoM/Apolipoprotein M |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55937 |
| Subcellular location | Secreted |
| Synonyms | Apo M; Apo-M; Apolipoprotein M; ApoM; APOM_HUMAN; G3A; HSPC336; MGC22400; NG20; NG20 like protein; Protein G3a. |
| Background | Apolipoproteins are protein components of plasma lipoproteins. ApoM (Apolipoprotein M), also known as protein G3a, is a member of the Lipocalin family of proteins. ApoM is exclusively expressed in kidney tubular epithelial cells and liver hepatocytes. Mature ApoM retains its signal peptide, which acts as a hydrophobic anchor, and contains a structurally conserved eight stranded antiparallel barrel which binds retinol and retinoic acid. ApoM may play a key role in reverse cholesterol transport. It mainly associates with high density lipoprotein (HDL) and to a lesser extent with triglyceride-rich lipoprotein (TGRLP) and low-density lipoprotein (LDL). ApoM is important for the pre-HDL formation. Pre-HDL is an important acceptor of peripheral cellular cholesterol. The concentration of ApoM in plasma strongly correlates with total cholesterol. Low concentrations of ApoM in plasma is associated with diabetes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ApoM Polyclonal Antibody, Cy5.5 Conjugated (BS-12501R-CY5-5) validated for, and at what dilution?
BS-12501R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ApoM Polyclonal Antibody, Cy5.5 Conjugated (BS-12501R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse.
How should BS-12501R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ApoM Polyclonal Antibody, Cy5.5 Conjugated (BS-12501R-CY5-5)?
KLH conjugated synthetic peptide derived from human ApoM/Apolipoprotein M
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12501R-CY5-5)?
Cy5.5 on BS-12501R-CY5-5: excitation / emission 675nm/694nm.