API5 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1252R-Cy3-100uL |
| Product Name | API5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Guinea Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human API5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8539 |
| Swiss Prot | Q9BZZ5 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | AAC11; AAC-11; Apoptosis inhibitor 5; API-5; Antiapoptosis clone 11 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL; API5; MIG8 |
| Background | Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is API5 Polyclonal Antibody, Cy3 Conjugated (bs-1252R-Cy3) validated for, and at what dilution?
bs-1252R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does API5 Polyclonal Antibody, Cy3 Conjugated (bs-1252R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Guinea Pig.
How should bs-1252R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise API5 Polyclonal Antibody, Cy3 Conjugated (bs-1252R-Cy3)?
KLH conjugated synthetic peptide derived from human API5
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1252R-Cy3)?
Cy3 on bs-1252R-Cy3: excitation / emission 512,550nm/570,615nm.