Vimentin (Ser55) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12757r-rbitc-100uL |
| Product Name | Vimentin (Ser55) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser55 |
| Modification Site | Ser55 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7431 |
| Swiss Prot | P08670 |
| Subcellular location | Cytoplasm |
| Synonyms | Vimentin (phospho S55); p-Vimentin (phospho S55); CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; vimentin. |
| Background | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009] |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Vimentin (Ser55) Polyclonal Antibody, RBITC Conjugated (bs-12757R-RBITC) validated for, and at what dilution?
bs-12757R-RBITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Vimentin (Ser55) Polyclonal Antibody, RBITC Conjugated (bs-12757R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Horse.
How should bs-12757R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Vimentin (Ser55) Polyclonal Antibody, RBITC Conjugated (bs-12757R-RBITC)?
KLH conjugated synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser55
What are the excitation and emission wavelengths of the RBITC conjugate (bs-12757R-RBITC)?
RBITC on bs-12757R-RBITC: excitation / emission 570nm/595nm.