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VPS29 Polyclonal Antibody, HRP Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • Zebrafish
Overview
Catalog # bs-12770R-HRP-100uL
Product Name VPS29 Polyclonal Antibody, HRP Conjugated
Applications
ELISA IHC-P IHC-F
Specificity 91% homology with Rat VPS29 Protein
Reactivity Mouse
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human VPS29
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51699
Swiss Prot Q9UBQ0
Subcellular location Cytoplasm, Cell membrane
Synonyms DC15; DC7; DKFZp564F0223; FLJ20492; hVPS29; PEP11; PEP11 homolog; Retromer protein; Vacuolar protein sorting 29; Vacuolar protein sorting 29 (yeast homolog); Vacuolar protein sorting 29 (yeast); Vacuolar protein sorting 29 homolog (S. cerevisiae); Vacuolar protein sorting 29 homolog; Vacuolar protein sorting associated protein 29; Vacuolar protein sorting-associated protein 29; Vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; VPS 29; VPS29; VPS29_HUMAN; x 007 protein.
Background This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013]
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is VPS29 Polyclonal Antibody, HRP Conjugated (bs-12770R-HRP) validated for, and at what dilution?

bs-12770R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does VPS29 Polyclonal Antibody, HRP Conjugated (bs-12770R-HRP) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish.

How should bs-12770R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise VPS29 Polyclonal Antibody, HRP Conjugated (bs-12770R-HRP)?

KLH conjugated synthetic peptide derived from human VPS29

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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