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WAC Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-12787R-Biotin-100uL
Product Name WAC Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human WAC
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 51322
Swiss Prot Q9BTA9
Synonyms 1110067P07Rik; BA48B24; BA48B24.1; BM 016; FLJ31290; KIAA1844; MGC10753; OTTHUMP00000019380; OTTHUMP00000019382; PRO1741; wac; WAC_HUMAN; WW domain containing adapter protein with coiled coil; WW domain containing adaptor with coiled coil; WW domain-containing adapter protein with coiled-coil; Wwp4.
Background Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is WAC Polyclonal Antibody, Biotin Conjugated (bs-12787R-Biotin) validated for, and at what dilution?

bs-12787R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does WAC Polyclonal Antibody, Biotin Conjugated (bs-12787R-Biotin) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-12787R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise WAC Polyclonal Antibody, Biotin Conjugated (bs-12787R-Biotin)?

KLH conjugated synthetic peptide derived from human WAC

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