IKB alpha Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1287R-FITC-100uL |
| Product Name | IKB alpha Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse IKB alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 18035 |
| Swiss Prot | Q9Z1E3 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Nfkbi; AI46215; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Nfkbia; Ikba |
| Background | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IKB alpha Polyclonal Antibody, FITC Conjugated (bs-1287R-FITC) validated for, and at what dilution?
bs-1287R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IKB alpha Polyclonal Antibody, FITC Conjugated (bs-1287R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1287R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IKB alpha Polyclonal Antibody, FITC Conjugated (bs-1287R-FITC)?
KLH conjugated synthetic peptide derived from mouse IKB alpha
What are the excitation and emission wavelengths of the FITC conjugate (bs-1287R-FITC)?
FITC on bs-1287R-FITC: excitation / emission 494nm/518nm.