ADAP/FYB Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13236R-Cy5.5-100uL |
| Product Name | ADAP/FYB Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FYB/ADAP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2533 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ADAP; Adhesion and degranulation promoting adaptor protein; FYB 120/130; Fyb; FYB-120/130; FYB_HUMAN; FYN binding protein; FYN T binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP 130; SLAP-130; SLAP130; SLP 76 associated phosphoprotein; SLP-76-associated phosphoprotein; SLP76 associated phosphoprotein. |
| Background | Fyb (Fyn binding protein) and the anchoring proteins SKAP55 (src kinase-associated phosphoprotein) and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn (13). SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells (13). SKAP55 contains an amino-terminal pleckstrin homology domain and a carboxy-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY) (4,5). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain (1,2). SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation (6). The human Fyb gene maps to chromosome 5p13.1 and encodes a 783 amino acid protein (7). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ADAP/FYB Polyclonal Antibody, Cy5.5 Conjugated (BS-13236R-CY5-5) validated for, and at what dilution?
BS-13236R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-13236R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ADAP/FYB Polyclonal Antibody, Cy5.5 Conjugated (BS-13236R-CY5-5)?
KLH conjugated synthetic peptide derived from human FYB/ADAP
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-13236R-CY5-5)?
Cy5.5 on BS-13236R-CY5-5: excitation / emission 675nm/694nm.